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Changes in the DNA ploidy patterns of human colorectal carcinomas, subsequent to culture or xenografting
Author(s) -
McA. Foster Hamish,
Tay Diana L. M.,
Whitehead Robert H.
Publication year - 1990
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930450103
Subject(s) - ploidy , flow cytometry , dna , cell culture , carcinoma , colonic carcinoma , biology , cell , microbiology and biotechnology , cancer research , pathology , medicine , genetics , gene
DNA ploidy patterns were measured by flow cytometry in 7 human carcinomas and compared with the DNA histograms of 3 cell lines and 25 xenografts and derived from them. Four of the carcinomas showed DNA ploidy changes on initial xenografting or early passaging, while 2 carcinomas showed stable ploidy patterns over several xenograft passages. One xenograft derived from a cell line showed a marked, but stable, increase in DNA content over the original human carcinoma. Two of the 3 cell lines were DNA aneuploid, and at least 1 line was apparently derived from a minor subpopulation in the original carcinoma. The possible mechanisms and implications of these changes are discussed.