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Effects of hyperthermia and irradiation on the growth of mouse melanoma tumors following immunotherapy
Author(s) -
Green Daniel M.,
Samulski Thaddeus V.,
Vervaert Carol,
Halperin Edward C.,
Oleson James R.,
Seigler Hilliard
Publication year - 1990
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930440413
Subject(s) - medicine , melanoma , hyperthermia , radiation therapy , immunotherapy , adjuvant , ionizing radiation , cancer research , pathology , irradiation , oncology , nuclear medicine , cancer , physics , nuclear physics
Recent investigations suggest that adjuvant hyperthermia enhances the response of human malignant melanoma to ionizing radiation. A study was undertaken to explore the effect of radiation and hyperthermia on the responsiveness of melanoma following active immunization with lethally irradiated B16 melanoma cells in C57 BL 6 mice. Two groups of mice were treated: group 1 received immunotherapy on days 2–5 following tumor innoculation, and group 2 received immunotherapy on days 15–18 following tumor innoculation. The mice in each group were subsequently randomized into one of three subgroups: 1) no further treatment; 2) radiation therapy alone (6 Gy in a single fraction); 3) radiation therapy and regional hyperthermia (43°C × 1 hour). Tumor sizes were measured regularly, and between day 52 and 63 the animals were sacrificed and the lungs sectioned for counting of metastatic tumors. Results from this study show that the immunomodulated B16 murine melanoma is responsive to radiation therapy alone and that this responsiveness is enhanced by the addition of adjuvant regional hyperthermia. There did not appear to be any significant effect of radiation therapy or radiation therapy plus hyperthermia on the development of lung metastases in this tumor model system.