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Inhibition of the growth of human melanoma xenografts in nude mice by human tumor‐specific cytotoxic T‐cells
Author(s) -
Crowley Nancy J.,
Slingluff Craig L.,
Vervaert Carol E.,
Darrow Timothy L.,
Seigler Hilliard F.
Publication year - 1990
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930430202
Subject(s) - cytotoxic t cell , melanoma , in vivo , medicine , in vitro , immunotherapy , cancer research , immunology , biology , immune system , biochemistry , microbiology and biotechnology
Melanoma‐specific T‐cells (CTLs) are specifically cytotoxic for autologous tumor, when assayed in vitro. To examine their effectiveness in vivo, we tested the ability of these human T‐cells to inhibit growth of human melanoma xenografts by using a Winn assay. Nude mice receiving specific CTLs (n = 10) demonstrated a dramatic inhibition of tumor growth. All treated mice were tumor‐free at day 50 and nine remained tumor‐free at day 65, vs. control mice (n= 10) with average tumor volumes of 321 mm 3 and 808 mm 3 , respectively. To control for the possibility that a non‐specific response to the human T‐cells could inhibit tumor growth, an additional group received allospecific CTLs. There was no inhibition of tumor growth in this group (n =8), with the average tumor volume of 2,768 mm 3 at day 40 vs. 1,882 mm 3 in the control group (n = 10). We conclude that these tumor‐specific CTLs can inhibit tumor growth in vivo and may prove useful in the adoptive immunotherapy of melanoma.