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Flow cytometric DNA content analysis in primary lung cancer: Comparison of results from fresh and paraffin‐embedded specimens
Author(s) -
Isobe Hiroshi,
Miyamoto Hiroshi,
Inoue Kazuaki,
Shimizu Mikio,
Endo Takashi,
Mizuno Shigetaka,
Kawakami Yoshikazu
Publication year - 1990
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930430110
Subject(s) - dna , pathology , flow cytometry , lung cancer , ploidy , medicine , microbiology and biotechnology , biology , gene , genetics
Tumor DNA content was determined by flow cytometry in 24 surgically resected specimens from patients with primary lung cancer to compare results from fresh and paraffin‐embedded specimens. We found a good correlation in DNA indices between fresh and paraffin‐embedded specimens ( r =.978), although one tumor with DNA near‐diploidy was mistaken as DNA diploid in paraffin‐embedded specimens. In the measurements of paraffin‐embedded specimens, eight of 24 tumors were DNA multiploid and showed DNA content heterogeneity. These results indicate that DNA content analysis of paraffin‐embedded specimens is helpful because it makes it easy to analyze multiple specimens from different parts of the tumor.