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Improvement of the rapid immunoperoxidase staining method for intraoperative pathological diagnosis of pancreatic cancer using microwave irradiation
Author(s) -
Ichihara Tohru,
Nakao Akimasa,
Suzuki Yuichi,
Sakamoto Junichi,
ami Toshiaki,
Harada Akio,
Nagura Hiroshi,
Takagi Hiroshi
Publication year - 1989
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930420316
Subject(s) - medicine , frozen section procedure , immunostaining , pancreatic cancer , pathology , immunoperoxidase , staining , biopsy , cancer , pathological , antigen , monoclonal antibody , immunohistochemistry , antibody , immunology
The rapid immunostaining of gastrointestinal cancer‐associated antigens, CA19–9, CEA, DUPAN2, and CA50, for intraoperative pathological diagnosis of pancreatic cancer can be completed in only 13 min by applying microwave irradiation, which was used to accelerate the incubation of the primary antibody. Only 3 s irradiation at 500 W for fresh‐frozen sections produced specific antigen staining of greater intensity than that obtained with longer incubation, and tissue preservation was satisfactory with minimal non‐specific background staining. This rapid immunoperoxidase staining method on the cryostat section of pancreatic biopsy specimens can provide important information for determining an appropriate operative method for pancreatic cancer patients.