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Colon ornithine decarboxylase activity following standard endoscopy preparation regimens
Author(s) -
Love Richard R.,
Verma Ajit K.,
Surawicz Tanya S.,
Morrissey John F.
Publication year - 1989
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930420304
Subject(s) - ornithine decarboxylase , rectum , enema , medicine , ornithine , colorectal cancer , gastroenterology , putrescine , sigmoid colon , adenoma , polyamine , pathology , endocrinology , cancer , biochemistry , enzyme , biology , amino acid , arginine
Ornithine decarboxylase (ODC) catalyzes the formation of putrescine from ornithine, which is the first step in the pathway of mammalian polyamine biosynthesis. Tissue activity levels of ODC have been suggested to be a marker of risk for colorectal cancer in hereditary polyposis and in adenoma formers. We analyzed ODC activity in rectal and sigmoid colon mucosal biopsies obtained at 10 cm and at 30 cm in 40 healthy, colon cancer risk factor‐free adults following three endoscopic preparation regimens: (1) no special preparation; (2) two phosphate enemas; and (3) “Colyte” lavage preparation 12 hr previously. Levels of ODC, measured in fresh tissue, were approximately twofold higher for enema preparation vs. no preparation (for log‐transformed data: sigmoid, P < 0.0001; rectum, P = 0.0001) and for enema preparation vs. lavage (sigmoid, P = 0.0002; rectum, P = 0.008). Lavage and no preparation ODC levels were not significantly different. ODC activity levels ranged from 0.00 to 352.96 pmol/mg/hr.