Premium
Application of the immunoperoxidase method for rapid intraoperative pathological diagnosis of pancreatic cancer
Author(s) -
Ichihara Tohru,
Nakao Akimasa,
Sakamoto Junichi,
ami Toshiaki,
Harada Akio,
Watanabe Tadashi,
Takagi Hiroshi,
Nagura Hiroshi
Publication year - 1989
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930400105
Subject(s) - immunostaining , medicine , pathology , staining , frozen section procedure , h&e stain , biopsy , cancer , pancreatic cancer , immunoperoxidase , carcinoma , histopathology , immunohistochemistry , antibody , monoclonal antibody , immunology
Intrapancreatic extension of pancreatic duct cell carcinoma in 19 resectable cases was evaluated by both hematoxylin and eosin (H&E) staining and quick immunostaining using antibodies against cancer‐associated antigens —e.g., CA19‐9, DUPAN 2, and CEA—on the intraoperative frozen‐section biopsy during the course of laparotomy. By H&E staining, malignant lesions were identified in four of 19 cases (21%). By the immunostaining method, however, cancer cells were detected in 10 of 19 cases (52.6%). This immunostaining method was particularly useful in defining cancer cells surrounded by dense fibrous connective tissue, in which cancer cells are usually difficult to evaluate by H&E staining. Thus, the application of such immunostaining, together with H&E staining, on the cryostat sections of biopsy specimens may provide important information for the appropriate operative method. Retrospective study of permanent sections revealed that pathological diagnosis by quick immunostaining was more accurate than diagnosis by H&E staining.