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Effects of rat platelet extracts and human platelet‐derived growth factor on megakaryocytopoiesis in vitro
Author(s) -
Uyar Ruhi,
Cooper George W.,
Hou Xiao Ping
Publication year - 1988
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930390402
Subject(s) - megakaryocytopoiesis , megakaryocyte , platelet , in vitro , platelet derived growth factor receptor , growth factor , stimulation , medicine , platelet derived growth factor , platelet rich plasma , endocrinology , andrology , immunology , haematopoiesis , biology , microbiology and biotechnology , biochemistry , receptor , stem cell
An improved plasma clot culture system was developed for the culture of rat megakaryocyte colonies in vitro. Greater stimulation of megakaryocyte colony growth was provided when both serum and plasma were added to the culture medium than when plasma was added alone. Freeze‐thawed extracts prepared from 900 × 10 6 platelets/ml saline and also 9 ng/ml of human platelet‐derived growth factor (PDGF) caused maximum increases over the controls of 71% and 42%, respectively, in the numbers of megakaryocyte colonies. The increase in the numbers of megakaryocyte colonies induced by platelet extracts and by PDGF implied that substance(s) released by platelets might play an important role in the control of megakaryocytopoiesis.