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Thermal resistance of human malignant melanoma modulated by prostaglandin E 2
Author(s) -
Kern David H.,
Krag David N.,
Kauffman Gordon L.,
Morton Donald L.,
Storm F. Kristian
Publication year - 1988
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930370117
Subject(s) - prostaglandin e2 , thymidine , melanoma , prostaglandin , cell culture , dna synthesis , dna damage , medicine , prostaglandin e , cancer research , cell , dna , microbiology and biotechnology , biochemistry , biology , genetics
The inhibition of DNA synthesis in a human malignant melanoma cell line as measured by tritiated thymidine ( 3 H‐TdR) incorporation was both time and temperature dependent. Two components of cell damage were identified: a cytostatic, temporary component from which no was observed. Thermotolerance was induced in M14 cells by sublethal heat treatment at 41°C for 1 hr. However, induction of thermotolerance was blocked by indomethacin, a prostaglandin synthetase inhibitor. Exogenous PGE 2 at concentrations up to 10 μg/ml also protected cells from heat damage. These data suggest that prostaglandin synthesis increases during heat stress and may play a role in protecting cells from thermal damage.