Premium
Effects of administration routes on the uptake of uridine and 5‐fluorouridine into an adenocarcinoma transplanted to rat liver
Author(s) -
Erichsen Christian,
Christenson PerInge,
Eriksson Göran,
Yngner Torsten,
Jönsson PerEbbe,
Stenram Unne
Publication year - 1986
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930330203
Subject(s) - uridine , medicine , peritoneal cavity , systemic administration , portal vein , floxuridine , rna , femoral vein , pharmacology , endocrinology , pathology , biochemistry , cancer , surgery , fluorouracil , biology , in vivo , microbiology and biotechnology , gene
In a model of secondary liver cancer in Wistar rats, the effect of different administration routes on the uptake of 3 H‐uridine into tumor and several normal tissues was studied. The rats were inoculated with a tumor cell suspension in the central liver lobe. Ten days later, they were distributed into four groups with a catheter placed in the gastroduodenal artery, the portal vein, one of the femoral veins, or in the peritoneal cavity. 3 H‐uridine was injected 46 h later and after an additional 90 minutes the animals were anesthezised and pieces of liver tumor and normal tissues were removed and frozen. The incorporation into the acid‐soluble fraction and RNA was analyzed. In a separate experiment, 3 H‐fluorouridine was administered by the gastroduodenal artery and a comparison was made with the uptake of 3 H‐uridine. A significantly higher amount of uridine was incorporated into the tumor by me arterial route. The intraportal and intraperitoneal routes were comparable, while a somewhat higher incorporation was found by the systemic route. The consequences of using the different routes upon the incorporation into RNA of tumor and dose‐limiting organs are demonstrated. With the aid of this experimental model, it is possible to evaluate further the effect by different manipulations on different drugs regarding the administration route.