Premium
Colorectal carcinoma in a rat model: Suppression of tumour development and altered host immune status following treatment with anti B‐lymphocyte serum
Author(s) -
House A. K.,
Maley M. A. L.
Publication year - 1986
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930320416
Subject(s) - medicine , lymphocyte , immune system , mesenteric lymph nodes , spleen , in vivo , saline , lymph node , 1,2 dimethylhydrazine , in vitro , immunology , endocrinology , colorectal cancer , biology , cancer , dimethylhydrazine , biochemistry , microbiology and biotechnology
The rate of colonic tumour development and immune capability in rats whose B‐lymphocyte function was suppressed by injections of rabbit anti‐rat IgM and also given the carcinogen dimethylhydrazine (DMH) were studied. Four rat groups were arranged to receive either DMH + anti ‐ IgM, DMH + normal rabbit serum (NRS), saline + anti‐IgM, or saline + NRS. Tumour weight, blood and mesenteric lymph node B‐lymphocyte numbers, in vivo allograft response, in vitro lymphocytotoxicity, and leucocyte migration inhibition response (LMI) were recorded fortnightly. Tumour induction was delayed in the DMH + anti‐IgM (treated tumour) group, which developed less tumour than the DMH + NRS (untreated tumour) group (p < 0.001). Spleen cell lymphocytotoxicities were depressed in treated rats when compared to either the saline+anti‐IgM (treated control) rats or the untreated rats (P < 0.02), whereas anti‐IgM treatment suppressed lymphocytotoxicity responses in control rats (p < 0.05). The untreated tumour rats were tumour immune by LMI; however, The treated tumour rats did not express this in vitro tumour immunity. The B‐lymphocyte levels in the mesenteric lymph nodes of untreated tumour rats increased with tumour induction (p < 0.05), whereas in the treated tumour rats B‐lymphocyte levels were not similarly stimulated.