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Comparison of specific red‐cell adherence and immunoperoxidase staining techniques for ABO(H) blood‐group cell‐surface antigens on superficial transitional cell carcinoma of the bladder
Author(s) -
Boileau Michel A.,
Cowles Robert S.,
Schmidt Karmen L.,
Schmidt Waldemar A.
Publication year - 1985
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930300203
Subject(s) - immunoperoxidase , abo blood group system , antigen , pathology , medicine , staining , cell , transitional cell carcinoma , immunology , biology , antibody , monoclonal antibody , cancer , bladder cancer , genetics
The presence or absence of blood‐group antigens have been used to predict the clinical course of patients with superficial transitional cell carcinoma of the bladder. Antigen loss has been associated with neoplastic change. The red‐cell adherence test has been the most widely accepted method of antigen determination, but this technique has inherent weaknesses. Recently, the immunoperoxidase assay has been used to detect antigens on tumor cells. We compared 30 patients using the red‐cell adherence and immunoperoxidase methods on adjacent micro to me cut sections. The red‐cell adherence and immunoperoxidase methods performed similarly (86 %) when assessing antigen presence or absence. However, the immunoperoxidase method was clearly superior in: 1) specificity for antigens on tumor cells and normal internal controls; 2) localization of antigen; 3) demonstration of cellular morphology; 4) increased objectivity of analysis; 5) ease of reproducibility; and 6) cost effectiveness.