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An enzymatic method for the consistent production of monodispersed viable cell suspensions from human solid tumors
Author(s) -
Rong Guo Huang,
Grimm Elizabeth A.,
Sindelar William F.
Publication year - 1985
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930280213
Subject(s) - collagenase , hyaluronidase , enzyme , sarcoma , pathology , cell , deoxyribonuclease , medicine , cancer research , chemistry , biochemistry
An enzymatic method is described for disaggregation of viable tumor cells from human solid tumors. The enzymatic cocktail consists of 0.1 % collagenase, 0.01% hyaluronidase, and 0.002% deoxyribonuclease. After mechanical mincing of the tumor tissue, tumor specimens are dissociated by incubation in the enzymatic cocktail for 12–18 hours at room temperature. In 17 cases of sarcoma, the mean yield was 5 × 10 6 viable cells per gram tumor tissue. Yield was 1 × 10 7 viable cells per gram tumor tissue in 23 cases of gastrointestinal carcinoma. The viabilities of tumor cell suspensions ranged from 50 to 98%, except for low viabilities in four specimens that were grossly composed almost entirely of necrotic tissue. The dissociation procedure is simple and the viable cell yield is sufficient for applications in studies of human cancer immunobiology.