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Fluorescent histochemical demonstration of estrogen and progesterone binding in giant cell tumors of bone: Preliminary observations
Author(s) -
Malawer Martin,
Bray Michael,
Kass Mary
Publication year - 1984
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930250303
Subject(s) - estrogen , cytoplasm , estrogen receptor , stromal cell , progesterone receptor , cytosol , receptor , staining , endocrinology , medicine , hormone , chemistry , pathology , breast cancer , cancer , biochemistry , enzyme
Hormonal receptors of giant cell tumors (GCTs) have not been previously reported. Five cases of GCT of bone were analyzed for estrogen and progesterone binding. Frozen sections were studied by a histochemical method, using 17‐beta‐6‐CMOBSA‐FITC and 11‐alpha‐hydroxyprogester‐one‐HS‐BSA‐TMRITC. Cytoplasmic fluorescence with estradiol‐BSA‐FITC was seen in stromal cells of all five tumors; three also showed cytoplasmic staining with progesterone‐BSA‐TMRITC. No fluorescence was seen in the giant cells. Two cases were also analyzed by a dextrancoated charcoal cytosol assay. No estrogen or progesterone receptor was detected by cytosol assay. This study suggests that the estrogen and progesterone binding is present in GCT of bone, possible to low‐affinity sites rather than high‐affinity receptors. Further investigation of hormonal receptors of GCTs of bone is warranted.