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Proliferative potential of cells from normal human colon epithelium, adenomas, and carcinomas of the large bowel
Author(s) -
Agrez M. V.,
Barham S. S.,
Tarara J. E.,
Wolff B. G.,
Reiman H. M.,
Ilstrup D. M.,
Lieber M. M.
Publication year - 1983
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930240405
Subject(s) - flow cytometry , epithelium , pathology , malignancy , medicine , colorectal cancer , biology , cancer , immunology
The proliferative potential of nonmalignant and malignant cells obtained from the human large bowel was assessed with a soft agar culture colony‐forming technique and by flow cytometry to analyze cell cycle kinetics. No colony formation occurred with samples of normal human adult colon mucosa. The absence of colony formation in all but 1 of 24 evaluable colorectal adenomas suggested that malignancy of tissue in vivo is generally necessary for colony formation to occur in soft agar cultures. A high proliferative index (S + G 2 /M), as assessed by flow cytometry, was significantly associated with higher soft agar culture counts in a series of 25 colorectal carcinomas.