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Sensitivity of proliferating human breast epithelial cells to hypotonic treatment
Author(s) -
Goldstone Stephen E.,
Stanyon Roscoe,
Lan Sam
Publication year - 1982
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930210413
Subject(s) - radiosensitivity , tonicity , epithelium , medicine , fibroblast , pathology , andrology , cancer research , microbiology and biotechnology , biology , cell culture , radiation therapy , genetics
An assay for colony‐forming cells of breast epithelia derived from normal and malignant surgical specimens is described using an IMR 90 fibroblast feeder layer. Their radiosensitivity (Do: 120–172) is consistent with the proliferative origin of the colonies. Distilled water inhibits proliferation of a proportion of the colony‐forming cells after a 1‐minute exposure. Continued detection of colonies after 10 minutes of exposure indicates that it is an inefficient way of completely eradicating proliferating epithelial cells of normal and malignant origin.