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High‐flow isolation perfusion of the rat hind limb in vivo
Author(s) -
Benckhuysen C.,
Van Dijk W. J.,
Van Hoff S. C. T
Publication year - 1982
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930210412
Subject(s) - perfusion , in vivo , hindlimb , oxygen , medicine , blood flow , anatomy , chemistry , biology , microbiology and biotechnology , organic chemistry
An in vivo model for perfusion chemotherapy of the rat hind limb is described in which perfusion at a predetermined flow rate of up to 4 ml·min −1 can be rapidly established in a tourniquet‐isolated limb. Standard additions to the semisynthetic perfusion medium included glucose, insulin, and lactate from fresh human erythrocytes. Arteriovenous differences in oxygen content revealed a gradual increase with time of the rate of oxygen consumption until a plateau phase was reached. The plateau rates of oxygen consumption, and of utilization of lactate and of acetoacetate, when supplied, were significantly higher at 4 ml flow·min −1 than at 1.2 ml·min −1 . When perfusion was performed at 4 ml·min −1 , and suitable metabolic substrates were supplied, the rate of oxygen consumption at the plateau level was apparently equal to the estimated in vivo rate.