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Morphological studies of peripheral monocytes during in vitro maturation by scanning electron microscopy
Author(s) -
Krishnan Engikolai C.,
Me Cheruvillil D.,
Krishnan Leela,
Jewell William R.
Publication year - 1982
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930200214
Subject(s) - in vitro , medicine , peripheral , scanning electron microscope , electron microscope , pathology , microbiology and biotechnology , biology , optics , biochemistry , materials science , composite material , physics
Human peripheral monocytes were cultured in autologous serum over a period of 14 days. Morphological changes were observed in both light and scanning electron microscopy. Nonspecific esterase, Fc receptor, and phagocytic activites were studied using antibody‐coated sheep erythrocytes. Though the spreading of cytoplasm was observable in a light microscope, scanning electron microscopic pictures revealed several interesting changes during the culture period. The number of filopodia and microvilli increased as the duration in culture increased. At the end of 7 days, almost pure monocytes remained in culture. These cells were characteristically 98–100% nonspecific‐esterase positive, Fc‐receptor positive, and highly phagocytic. This technique may be of value in understanding changes due to various specific and nonspecific immune stimulators.