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Clonal growth of normal and malignant human breast epithelia
Author(s) -
Lan Sam,
Smith Helene S.,
Stampfer Martha R.
Publication year - 1981
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930180313
Subject(s) - fibroblast , pathology , malignant cells , linear growth , primary culture , mammary gland , human breast , medicine , cell culture , breast cancer , biology , cancer , genetics , mathematics
We have developed an efficient method for the growth of colonies from single breast epithelial cells derived from nonmalignant [6], primary [7], and metastatic [1] surgical specimens. The immediate sources of the cells were primary and secondary mass cultures. An enriched culture medium and fibroblast feeder layers were used. A linear relationship between the number of cells cultured and the number of colonies obtained was found. Efficiency of colony formation was higher for those derived from nonmalignant specimens (mean 24%) than those derived from malignant specimens (mean 11%). Fibroblast feeder layers increased the efficiency of colony formation and also supported extended growth of colonies.