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An in vitro clonal assay for bladder cancer: Studies of the biologic potential of the urothelium and determination of in vitro sensitivity to cytotoxic agents
Author(s) -
Stanisic T. H.,
Buick R. N.,
Trent J. M.,
Fry S. E.,
Salmon S. E.
Publication year - 1981
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930180111
Subject(s) - urothelium , bladder cancer , in vitro , medicine , clone (java method) , biopsy , pathology , cytotoxic t cell , agar , cancer research , urology , cancer , urinary bladder , biology , biochemistry , gene , bacteria , genetics
We have applied an in vitro colony‐forming assay system to the study of primary urothelial explants obtained transurethrally by bladder barbotage and tumor biopsy from 91 patients. Urothelial cells obtained by bladder barbotage from patients with bladder cancer and from a “control” group of individuals exhibit differential capacity to clone in agar. Of samples from bladder cancer patients, 88% formed colonies, whereas only 27% of these from a “control” group did so. Of samples from a “suspicious” patient group, 70% formed colonies. In vitro drug sensitivity studies of cells obtained by biopsy of solid tumors demonstrate a spectrum of drug sensitivity to cytotoxic agents.