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Adenosine‐aminohydrolase activity in the erythrocytes, lymphocytes, and plasma of healthy subjects and kidney transplant recipients
Author(s) -
Keogh Brian,
Pauly John,
Tritsch George,
Mittelman Arnold,
Murphy Gerald P.
Publication year - 1976
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930080508
Subject(s) - adenosine , immunosuppression , adenosine deaminase , medicine , immune system , in vivo , lymphocyte , in vitro , kidney , red blood cell , antigen , immunology , endocrinology , chemistry , biochemistry , biology , microbiology and biotechnology
Adenosine‐aminohydrolase activity in the red blood cells and lymphocytes of transplant patients were assayed by continuous flow analysis. This enzyme, which has been implicated in the ability of lymphocytes to mount an immune response, was evaluated in renal allografted patients immunosuppressed by azothioprine and methylprednisone. Red cell adenosine‐aminohydrolase activity was depressed in all patients when compared to nontreated healthy controls. Adenosine aminohydrolase activity was raised in the lymphocytes of the renal allografted patients. Renal transplant patients' in vitro lymphocyte reactivity to antigens, mitogens, or skin testing to specific antigens, confirmed the depressive effects of immunosuppression observed in vivo. We conclude from these studies that red cell adenosine‐aminohydrolase activity may be a more sensitive index of the state of immunoresponsiveness than the corresponding enzyme activity in the lymphocyte.