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In vitro granulocytic stem cell clonogenic capacity of marrow and spleen cells from mice infected with rauscher leukemia virus
Author(s) -
Koltun Leonard A.,
Broxmeyer Hal,
Lobue Joseph,
Fredrickson Torgny N.,
Gordon Albert S.,
Gallicchio Vincent
Publication year - 1976
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930080311
Subject(s) - clonogenic assay , spleen , bone marrow , in vitro , leukemia , virus , stem cell , medicine , immunology , biology , virology , microbiology and biotechnology , biochemistry
Bone marrow and spleen cells from early, midstage, and terminal Rauscher leukemia virus (RLV‐A)‐infected erythroleukemic mice were assessed for granulocyte stem cell (CFU‐c) clonogenic capacity in the semisolid agar culture assay. It was found that marrow CFU‐c concentrations exceeded normal in early stages of this erythroid disease but returned to near normal values during mid‐ and terminal phases. Splenic CFU‐c concentrations, on the other hand, were generally higher than control values for all stages of the disease. These results are discussed with reference to the pathogenesis of RLV‐A disease.