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Impaired cytotoxicity of lymphocytes from patients with cancer
Author(s) -
SuciuFoca Nicole,
Buda Joseph A.,
Herter Frederic,
Molinaro Anthony,
Broell John,
Reemtsma Keith
Publication year - 1976
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930080112
Subject(s) - cytotoxicity , cytotoxic t cell , medicine , cancer , in vitro , immunology , lymphocyte , homologous chromosome , cancer cell , cancer research , biology , biochemistry , gene
We have investigated the ability of peripheral blood lymphocytes from 57 cancer patients and from 54 normal controls to exert cytotoxic activity in vitro on allogeneic target cells by using a residual tritiated proline assay. Phytohemagglutin was added to the cultures for potentiating the reaction. The cytotoxic potential of lymphocytes from cancer patients was significantly lower than that of healthy controls. Increased survival of target cells was found in numerous reactions with patients' lymphocytes, probably reflecting a “feeder” effect. The source of plasma used for testing, i.e., autologous or pooled normal homologous plasma, did not affect the strength of cytotoxicity reactions displayed by lymphocytes from either normal or cancer patients. A lower reactivity was generally seen in patients with metastatic disease than in patients with no evidence of distant spread.