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Control of erythropoietin production during hypoxic hypoxia
Author(s) -
Harvey S. R.,
Mirand E. A.,
Back N.,
Murphy G. P.
Publication year - 1973
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930050413
Subject(s) - puromycin , cycloheximide , endogeny , protease , proteases , hypoxia (environmental) , protein biosynthesis , erythropoietin , biochemistry , medicine , endocrinology , pharmacology , biology , enzyme , chemistry , organic chemistry , oxygen
The control of erythropoietin (ESF) production in rats subjected to hypoxic hypoxia was investigated by studying the inhibition of endogenous ESF production by inhibitors affecting proteases and protein synthesis. The study revealed that the production of endogenous ESF was inhibited by protease inhibitors, Trasylol and epsilon amino caproic acid (EACA) and inhibitors affecting protein synthesis such as actinomycin–D, puromycin, and cycloheximide. The inhibitory effect of actinomycin–D on ESF production was dose dependent and markedly dimiished (threefold) in hepatectomized rats, whereas the inhibitory effects of Trasylol, EACA, and Step–I ESF (used as feedback inhibitor) were enhanced. These preliminary findings suggest that, during hypoxic stress, the control of ESF production may involve the interaction of a protease‐globulin substrate to produce ESF as well as the de novo synthesis of a protein or proteins involved in ESF production.