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Immunologic response to melanoma of sensitized perfused bone and spleen
Author(s) -
Nolan P.,
Norton L.,
Abernathy C.,
Pierce G.,
Eiseman B.
Publication year - 1972
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930040503
Subject(s) - spleen , melanoma , medicine , pathology , immunology , cancer research
A B16 mouse melanoma which will not grow in tissue culture was implanted subcapsularly in 12 isolated perfused pig spleens and subperiosteally in four perfused pig femurs. Tumor implants grew to a maximum 3‐mm size within five to seven days. Metastases developed within the substance of the spleen but not within bones during the 7 to 15 day perfusions. The protective effects of perfustate, concentrated perfusate cells, and suspensions of spleen cells were determined by injecting the fluid fractions simultaneously with melanoma in 25 mice. Concentrated perfusate cells and splenic cells from perfusions over 10 days characteristically prolonged the mean time of tumor appearance and mean survival time of mice inoculated with melanoma. The possible immunologic mechanisms responsible for such protection are discussed.