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Lactate dehydrogenase isoenzyme patterns of prostatic cancer and hyperplasia
Author(s) -
Flocks Rubin H.,
Schmidt Joseph D.
Publication year - 1972
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930040210
Subject(s) - isozyme , lactate dehydrogenase , cancer , hyperplasia , medicine , pathology , prostate cancer , lactic dehydrogenase , agarose gel electrophoresis , prostate , enzyme , microbiology and biotechnology , biochemistry , biology , dna
Electrophoresis using agarose and lactic acid dehydrogenase (LDH) film plus scanning fluorometry provide a simple, fast method for precise and accurate measurement of serum and tissue LDH isoenzymes. Via this method prostate tissue LDH isoenzy mes were determined for over 200 patients with either hyperplasia or cancer. Increased LDH isoenzyme V activities generally selected out the cancer patterns, whereas peak isoenzyme I values did not distinguish between the two groups as well. The LDH index (I/V) only confirmed the LDH V values. That the metabolic changes of neoplasia precede histologic changes is suggested by two patients in whom repeat biopsies demonstrated cancer following elevated isoenzyme V levels in tissue originally considered benign.