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Excretion of tritiated 3–2′ dimethyl‐4‐diphenylamine
Author(s) -
Gerfo Paul Lo,
Herter Frederic P.
Publication year - 1972
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930040111
Subject(s) - diphenylamine , urine , tritium , tritiated water , kidney , medicine , excretion , carcinogen , endocrinology , metabolism , chromatography , chemistry , biochemistry , organic chemistry , physics , nuclear physics
Tritiated 3–2′Dimethyl‐4‐diphenylamine was prepared by catalytic exchange and studies were carried out in Wistar‐Furth rats following subcutaneous injection. Blood levels reached their peak in 12 hours and this correlated closely with the peak levels of label in the urine and bile. Ninety per cent of labelled compound was excreted within 5 days, less than 3% of the tritium excreted was in the form of tritiated water. Sixty‐five per cent of excreted label appeared in the stools, and 35% in the urine of intact animals. In animals with bile fistula, 85% of the label appeared in the bile and 15% appeared in the urine. This data suggested recirculation of the compound and radioautographs were done at 10 and 20 hours after injection of labelled carcinogen, to see if there was any preferred site of absorption in the G.I. tract. None was found. Radioautographs failed to show any preferred localization of the label in the cells of the G.I. tract, liver, kidney, or bladder.