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Growth characteristics of the stilbestrol‐induced hamster kidney tumor
Author(s) -
Dekernion Jean B.,
Fraley Elwin E.
Publication year - 1971
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930030505
Subject(s) - stilbestrol , diethylstilbestrol , hamster , hormone , carcinogenesis , in vivo , kidney , medicine , estrogen , population , endocrinology , cancer research , cancer , biology , microbiology and biotechnology , environmental health
The stilbestrol‐induced hamster kidney tumor retained hormone dependence in vivo . In order to elucidate the mode and site of hormone stimulation, tumors of the twelfth in vivo transplant generation as well as newly induced kidney tumors were explained to monolayer cell cultures. Various concentrations of diethylstilbestrol failed to stimulate cell growth or prolong viability, and cells died after two to eight subcultures. This was not secondary to in vitro selection of a hormone‐independent cell population, since hormone was required for growth of tumor recycled from cell culture to animals. The presence of an intact stroma seems to be a requisite for estrogen tumorigenesis in the hamster kidney.