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Growth retardant properties of liver extract
Author(s) -
Henderson I. W. D.
Publication year - 1970
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930020306
Subject(s) - liver tissue , fire retardant , dna synthesis , dna , in vitro , biochemistry , growth inhibition , pharmacology , chemistry , biology , medicine , organic chemistry
In an attempt to relate the tissue growth retardant properties of crude cell sap preparations or chalones, to an effect by a compound of known chemical structure, an extract of normal rat liver known to contain a small five‐ or six‐carbon molecule with at least one carbonyl group has been tested against normal and neoplastic liver. The measured parameters, DNA, RNA, and protein syntheses, using radioactive precursors were those closely concerned with replication and growth. Inhibition of all three syntheses were shown. In normal tissue the inhibition after a single dose lasted for 3 hr, after which the tissue returned to control values. In the case of the Novikoff hepatoma, the values returned to control levels after 1 hr. Analysis of data related to varying concentrations and times demonstrates maximal inhibition at a low concentration range. At high concentrations there is less inhibition. The kinetic plot suggests simple tissue degradation. At −20 C the extract is stable for 3 weeks. In aqueous solution at 0 C, activity is lost after a few days.