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In vivo response of mouse lymphocytes to phytohemagglutinin and colchicine
Author(s) -
Killen Duncan A.
Publication year - 1970
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930020207
Subject(s) - colchicine , peripheral blood mononuclear cell , concomitant , in vivo , medicine , peripheral , peripheral blood , immunology , lymphocyte , in vitro , biology , biochemistry , microbiology and biotechnology
The response of peripheral mononuclear leukocytes and the systemic tolerance of mice to parenterally administered phytohemagglutinin and colchicine were assessed in 200 animals. The administration of 0.5ml of a solution of purified phytohemagglutinin resulted in the appearance of seemingly immature and significantly enlarged ( p <0.005) peripheral mononuclear leukocytes. The appearance of large lymphocytes in peripheral blood was interpreted as evidence for the occurrence of lymphoblastic transformation in vivo . However, no blast cells were observed in the peripheral blood smears. The concomitant administration of colchicine prevented enlargement of the circulating mononuclear lymphocytes. The phytohemagglutinin effect was apparently negated or minimized by the concomitant administration of colchicine. The significance of this observation is unknown.