Premium
Correlation of urinary isoenzymes in baboon renal allografts and autografts
Author(s) -
Groenewald J. H.,
Bersohn I.,
Van Zyl J. A.,
Schoonees R.,
Weber H. W.,
Retief C. P.,
Murphy G. P.
Publication year - 1969
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930010207
Subject(s) - urinary system , baboon , medicine , kidney , urology , urine , renal function , isozyme , enzyme , alkaline phosphatase , endocrinology , pathology , biochemistry , biology
Urinary and blood isoenzymes and other biochemical tests were performed on fresh or fresh‐frozen specimens from intact renal‐allografted or renal‐autografted adult male and female Chacma baboons. Although elevations in urinary and blood LDH and alkaline phosphatase activity were noted, these failed to prove consistent, or to be specific indices of renal rejection, or to correlate with the level of measured renal function. LDH urinary enzymes as determined by paper electrophoresis disclosed a heretofore undescribed shift from a high, fast‐moving fraction (I) in renal‐allografted or renal‐autografted animals. The shift is therefore not related to renal rejection, but more to nonspecific ischemic cellular destruction. Techniques of starch gel and paper urinary isoenzyme measurements are compared, and the necessity of fresh catheterized specimens for accurate enzymatic determination is stressed.