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Identification of ampullary carcinoma mixed subtype using a panel of six antibodies and its clinical significance
Author(s) -
Liu Fangfang,
Shen Danhua,
Ma Yingteng,
Song Qiujing,
Wang Hanlin
Publication year - 2019
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.25311
Subject(s) - medicine , cdx2 , immunohistochemistry , ampulla of vater , subtyping , muc1 , carcinoma , pathology , gastroenterology , oncology , biology , gene expression , biochemistry , gene , computer science , homeobox , programming language
Objectives To investigate the function of immunomarkers CK7, CK20, CK17, CDX2, MUC1, and MUC2 in the identification of primary ampullary carcinoma mixed subtype. Methods Forty‐two cases of primary ampullary carcinoma were performed by immunohistochemical studies. The correlation between the mixed subtype and the other two subtypes and patient survival data was analyzed using the SPSS 16.0 statistical software. Results Among 42 cases, 12 (28.6%) cases were classified as mixed subtype, which showed variable expression patterns: 91.7% (11/12) for CK7, 83.3% (10/12) for CK20; 66.7% (8/12) for CK17, CDX2, and MUC1; and 50% (6/12) for MUC2. Ten (83.3%) mixed types coexpressed four or more immunomarkers. Eight (19%) intestinal subtypes mainly showed a positive expression of CK20, CDX2, and MUC2. Twenty‐two (52.4%) pancreaticobiliary subtypes showed a positive expression of CK7, MUC1, and CK17. Stages III and IV diseases in mixed subtype (25%) and intestinal subtype (25%) were less than pancreaticobiliary subtype(63.6%) ( p = 0.039). Follow‐up data appeared to show a better survival rate for patients with mixed subtype than those with pancreaticobiliary subtypes. Conclusion Immunohistochemical staining provided a more reliable means of diagnosing mixed ampulla carcinoma. Accurate subtyping of ampullary carcinoma is clinically important to select effective chemotherapy regimens and to assess disease prognosis.