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Could OX40 agonist antibody promote activation of the anti‐tumor immune response in gastric cancer?
Author(s) -
Martins Mário R.,
Santos Rogério L. d.,
Jatahy Kleber d. N.,
Matta Marina C. d.,
Batista Thales P.,
Júnior José Iran C.,
Begnami Maria D. F. S.,
Torres Leuridan C.
Publication year - 2018
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.25001
Subject(s) - immune system , medicine , immunology , immunotherapy , cancer , monocyte , flow cytometry , tumor necrosis factor alpha , receptor , cancer research , cancer immunotherapy , monoclonal antibody , antibody , t cell , peripheral blood mononuclear cell , cytotoxic t cell , antigen , biology , in vitro , biochemistry
Background and Objectives OX40, a membrane‐bound molecule of the tumor‐necrosis‐factor‐receptor superfamily, is a critical costimulatory receptor during the immune response, especially to T cells, but studies described their presence of OX‐40 on neutrophils and monocytes, suggesting a potential role in the activation of immune response. Our aim was to characterize costimulatory receptors OX40 expression on circulating leukocytes in gastric cancer to identify novel targets for immunotherapy. Methods Peripheral blood mononuclear cells were isolated from 24 gastric cancer patients and 34 healthy controls and the expression of costimulatory (OX40) receptors were analyzed on T cells, neutrophil and monocyte using monoclonal antibodies by flow cytometry. Results We found that the higher levels of OX40 + T cells, monocytes/OX40+ and neutrophils/OX40+ from gastric cancer patients when compared to controls ( P < 0.0001), and also higher levels of OX40+ T cells when compared to stages III and IV ( P = 0.02). Percentage levels of total T cells were similar between patients and controls. Conclusions OX40 as a therapeutic agent has been investigated in many preclinical tumor models. Our findings suggest that of levels of costimulatory in T cells in GC will direct future studies on the role that costimulatory receptors play in the failure of T cell‐mediated immunity.