z-logo
Premium
5‐fluorouracil combined with cisplatin and mitomycin C as an optimized regimen for hyperthermic intraperitoneal chemotherapy in gastric cancer
Author(s) -
Murata Satoshi,
Yamamoto Hiroshi,
Shimizu Tomoharu,
Naitoh Hiroyuki,
Yamaguchi Tsuyoshi,
Kaida Sachiko,
Takebayashi Katsushi,
Miyake Toru,
Tani Tohru,
Tani Masaji
Publication year - 2018
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.24906
Subject(s) - mitomycin c , cisplatin , medicine , chemotherapy , regimen , hyperthermic intraperitoneal chemotherapy , pharmacology , fluorouracil , etoposide , intraperitoneal injection , hyperthermia , drug , cancer , surgery , ovarian cancer , cytoreductive surgery
Background and Objectives Optimized drug regimens for hyperthermic intraperitoneal chemotherapy (HIPEC) have not been standardized completely in patients with advanced gastric cancer (GC). We evaluated an optimized anti‐tumor protocol comprising 5‐fluorouracil (5‐FU) combined with cisplatin (CDDP) and mitomycin C (MMC) in vitro for clinical use of HIPEC. Methods The sensitivities of 5‐FU, CDDP, or MMC, alone or in combination, using different drug concentrations, exposure times, and hyperthermic conditions (42°C) were determined in vitro by the CD‐DST method using 3 different differentiated GC cell lines. Results The tumor cell growth‐inhibitory effect of 5‐FU was concentration‐dependent for all cell lines. In addition, 5‐FU showed a hyperthermic sensitization effect at all drug concentrations for all cell lines. The appropriate concentration of each drug was 5‐FU, 200 µg/mL; CDDP, 10 µg/mL; MMC, 2 µg/mL. Under hyperthermic conditions, most growth‐inhibitory effects for each drug at 30 min was equivalent to 60 min of exposure; use of three drugs combined significantly inhibited growth compared with any of the drugs alone. Conclusion An appropriate in vitro intraperitoneal chemotherapy regimen for GC was combined use of 5‐FU, CDDP, and MMC at 42°C for 30 min.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here