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Downregulation of urinary cell‐free microRNA‐214 as a diagnostic and prognostic biomarker in bladder cancer
Author(s) -
Wang Jinfeng,
Zhang Xin,
Wang Lili,
Dong Zhaogang,
Du Lutao,
Yang Yongmei,
Guo Yuan,
Wang Chuanxin
Publication year - 2015
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.23937
Subject(s) - medicine , bladder cancer , biomarker , urine , urinary system , cancer , urology , urinary bladder , lymph node , oncology , biochemistry , chemistry
Background and objectives We aimed to investigate potential of urinary cell‐free microRNA‐214 (miR‐214) as a noninvasive biomarker for bladder cancer in this report. Methods We screened miR‐214 expression in medium from 2 bladder cancer cell lines to determine whether it is secretory. Then we validated expression of cell‐free miR‐214 in urine samples from an independent set of 192 preoperative bladder cancer patients, 80 matching postoperative patients and 169 healthy controls. Results miR‐214 was secreted from bladder cancer cell lines. Cell‐free miR‐214 levels were significantly attenuated in preoperative urine from bladder cancer patients, whereas its expression significantly increased in matched postoperative urine. Underexpressed extracellular miR‐214 in urine was significantly associated with higher tumor stage, higher lymph node status, higher grade, age and history of non‐muscle‐invasive bladder cancer (NMIBC). Urinary cell‐free miR‐214 could forcefully differentiate bladder cancer (area under the curve; AUC = 0.838; 95% CI = 0.796–0.875) patients from healthy controls. Additionally, miR‐214 in urine supernatant could serve as an independent prognostic predicator of recurrence‐free survival (RFS) and overall survival (OS) for patients with muscle‐invasive bladder cancer (MIBC). Conclusions Urinary cell‐free miR‐214 is a hopeful biomarker for tumor stratification, early diagnosis and prognostic assessment of bladder cancer. J. Surg. Oncol. 2015 111:992–999 . © 2015 Wiley Periodicals, Inc.