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In colon cancer, normal colon tissue and blood cells have altered telomere lengths
Author(s) -
VallsBautista Cristina,
PiñolFelis Carme,
ReñéEspinet Josep M.,
BuenestadoGarcía Juan,
ViñasSalas Joan
Publication year - 2015
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.23894
Subject(s) - medicine , colorectal cancer , telomere , pathology , colonoscopy , intestinal mucosa , cancer , gastroenterology , biology , dna , genetics
Background Telomere length (TL) shortened occurs in colorectal carcinogenetic process. Our objective is to determine if it is only a local fact or there are alterations in normal colon cells and in other body cells. Methods TL of tumoral and normal mucosa and leukocytes of 40 patients operated of colorectal cancer (CRC) and 40 control patients with normal colonoscopy were measured by Southern‐blot. Groups were matched by the same localization as tumors, sex, and age. Results In CRC patients, TRFL (Telomere Repeat Factor Length) leukocytes mean was 8.84 kpb, normal colonic mucosa 7.97 kpb, and tumoral mucosa 7.33 kpb ( P < 0.001). In the 40 normal control patients, mean TRFL of colonic mucosa was 7.76 kpb, while in blood cells was 7.01 kpb ( P < 0.001). We observed an inverse correlation between leukocytes TRFL and age (r 2 = 0.17, P = 0.008). Mucosa TRFL correlates significantly with patient's age (r 2 = 0.138, P = 0.018). TRFL of controls colonic mucosa correlates with TRFL of their blood cells (r 2 = 0.354, P < 0.001). Conclusions Normal colonic mucosa and leukocytes in CCR patients presents telomere altered in respect to normal patients. Telomere length in normal leukocytes could be an initial marker for colorectal cancer. J. Surg. Oncol. 2015 111:899–904 . © 2014 Wiley Periodicals, Inc.