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Clinical significance of SPARC gene expression in patients with gastric cancer
Author(s) -
Sato Tsutomu,
Oshima Takashi,
Yamamoto Naoto,
Yamada Takanobu,
Hasegawa Shinichi,
Yukawa Norio,
Numata Kazushi,
Kunisaki Chikara,
Tanaka Katsuaki,
Shiozawa Manabu,
Yoshikawa Takaki,
Akaike Makoto,
Rino Yasushi,
Imada Toshio,
Masuda Munetaka
Publication year - 2013
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.23425
Subject(s) - gene expression , medicine , cancer , clinical significance , extracellular matrix , matricellular protein , metastasis , gene , pathology , real time polymerase chain reaction , osteonectin , cancer research , immunohistochemistry , biology , genetics , enzyme , biochemistry , alkaline phosphatase , osteocalcin
Purpose Secreted protein acidic and rich in cysteine (SPARC) is one of the first known matricellular proteins that modulates interactions between cells and extracellular matrix. Recent studies investigated the clinical significance of SPARC gene expression in the development, progression, and metastasis of cancer. The present study examined the relations of the relative expression of the SPARC gene to clinicopathological factors and overall survival in patients with gastric cancer. Methods We studied surgical specimens of cancer tissue and adjacent normal mucosa obtained from 227 patients with previously untreated gastric cancer. The relative expression levels of SPARC mRNA in cancer tissue and in adjacent normal mucosa were measured by quantitative real‐time, reverse‐transcription polymerase chain reaction. Results The relative expression level of the SPARC gene was higher in cancer tissue than in adjacent normal mucosa. High expression levels of the SPARC gene were related to serosal invasion ( P = 0.046). Overall survival at 5 years differed significantly between patients with high SPARC gene expression and those with low expression ( P = 0.006). Conclusions Overexpression of the SPARC gene may be a useful independent predictor of outcomes in patients with gastric cancer. J. Surg. Oncol. 2013; 108:364–368 . © 2013 Wiley Periodicals, Inc.