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Abnormal expression of mi R ‐301a in gastric cancer associated with progression and poor prognosis
Author(s) -
Xu XiaoDong,
He XuJun,
Tao HouQuan,
Zhang Wei,
Wang YuanYu,
Ye ZaiYuan,
Zhao ZhongSheng
Publication year - 2013
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.23374
Subject(s) - cancer , microrna , cancer research , clone (java method) , metastasis , medicine , cancer cell , tumor progression , transfection , pathology , biology , cell culture , gene , biochemistry , genetics
Background and Objectives miR‐301a is significantly overexpressed in many cancers. However, its expression and biological role in gastric cancer remain poorly understood. We investigated microRNA‐301a (miR‐301a) expression in gastric cancer and determined its effects on cancer cell behavior and its clinical significance in the development and progression of gastric cancer. Methods We determined miR‐301a expression in gastric tumors and gastric cancer cell lines by reverse transcription‐polymerase chain reaction. The effects of miR‐301a on cell clone formation, migration, and invasion of HGC‐27 and SGC‐7901 cells were detected following transfection of an miR‐301a inhibitor. miR‐301a expression in a 304‐tissue gastric cancer microarray was determined by in situ hybridization and its role in progression and prognosis was analyzed. Results miR‐301a was upregulated in gastric tumor tissues and cell lines. Down‐regulation of miR‐301a significantly inhibited cell clone formation, migration, and invasion of HGC‐27and SGC‐7901 cells. Overexpression of miR‐301a in primary gastric cancer tissues was associated with tumor size, invasion depth, lymph node metastasis, and TNM stage. Conclusions miR‐301a overexpression correlated with TNM stage and prognosis, suggesting that miR‐301a is involved in cellular clone formation, migration, and invasion in vitro and may play an important role in the clinical progression and prognosis of gastric cancer. J. Surg. Oncol. 2013; 108:197–202 . © 2013 Wiley Periodicals, Inc.