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Novel focal adhesion protein kindlin‐2 promotes the invasion of gastric cancer cells through phosphorylation of integrin β1 and β3
Author(s) -
Shen Zhanlong,
Ye Yingjiang,
Kauttu Tuuli,
Seppänen Hanna,
Vainionpää Sanna,
Wang Shan,
Mustonen Harri,
Puolakkainen Pauli
Publication year - 2013
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.23353
Subject(s) - integrin , focal adhesion , angiogenesis , cell adhesion , cancer research , cancer , metastasis , medicine , cell cycle , cell growth , phosphorylation , cancer cell , adhesion , apoptosis , microbiology and biotechnology , cell , biology , chemistry , receptor , biochemistry , organic chemistry
Abstract Background We have found that the expression of the novel focal adhesion protein kindlin‐2 had a significant positive correlation with poor survival in gastric cancer. However, the mechanism by which kindlin‐2 acts in gastric cancer warrants further evaluation. Methods Kindlin‐2 mRNA expression in gastric cancer cell lines was measured by realtime RT‐PCR under normal and hypoxic conditions. Cell proliferation, apoptosis, cell cycle, tumor adhesion, cell invasion ability, and phosphorylation of integrin β1 and β3 proteins were measured to assess the influence of kindlin‐2 on the malignant behavior of gastric cancer cells. Results Kindlin‐2 mRNA expression was highest in the distant metastasis gastric cancer cell line Hs‐746T. Cell proliferation, adhesion with endothelium and collagen IV, invasion rate, and angiogenesis genes expression, as well as phosphorylation of integrin β1 and β3 in Hs‐746T, were decreased significantly after kindlin‐2 downregulation, but there was no change in apoptosis and cell cycle. Conclusions Kindlin‐2 might promote the invasion of gastric cancer cells through enhancing proliferation and adhesion by the phosphorylation of integrin β1 and β3. J. Surg. Oncol. 2013; 108:106–112 . © 2013 Wiley Periodicals, Inc.