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Overexpression of ZEB1 relates to metastasis and invasion in osteosarcoma
Author(s) -
Shen Aidong,
Zhang Yunqing,
Yang Huiguang,
Xu Ruisheng,
Huang Guowei
Publication year - 2011
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.23012
Subject(s) - osteosarcoma , transfection , metastasis , medicine , cancer research , carcinogenesis , cell culture , sarcoma , pathology , cancer , biology , genetics
Background This study aimed to investigate the expression of ZEB1 in osteosarcoma tissues and to discuss the relationship between ZEB1 expression and osteosarcoma metastasis. Methods Using RT‐PCR and Western blotting, the mRNA and protein expressions of ZEB1 in the osteosarcoma and normal bone tissues were detected. Using the RNA interference technique, the expression of ZEB1 in the human osteosarcoma MG‐63 cell line was downregulated, and the changes in the invasion of MG‐63 cells were examined. Results The positive mRNA expression rate of ZEB1 in the osteosarcoma tissues was significantly higher than that in normal bone tissue ( P < 0.05). The protein expression level of ZEB1 in the sarcoma tissues from patients with positive lung metastasis was significantly higher than that from patients without lung metastasis ( P < 0.05). After the transfection of ZEB1 siRNA into the MG‐63 cells, the protein expression of ZEB1 was significantly reduced ( P < 0.05), and the number of cells that passed through the Transwell chamber was significantly lower than that in the non‐transfected control group as well as the transfected control group ( P < 0.05). Conclusions The overexpression of ZEB1 in osteosarcoma may be related to the carcinogenesis and development as well as metastasis and invasion of osteosarcoma. J. Surg. Oncol. 2012; 105:830–834. © 2011 Wiley Periodicals, Inc.