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Oncofetal protein, IMP‐3, a potential marker for prediction of postoperative peritoneal dissemination in gastric adenocarcinoma
Author(s) -
Okada Kaoru,
Fujiwara Yoshiyuki,
Nakamura Yurika,
Takiguchi Shuji,
Nakajima Kiyokazu,
Miyata Hiroshi,
Yamasaki Makoto,
Kurokawa Yukinori,
Takahashi Tsuyoshi,
Mori Masaki,
Doki Yuichiro
Publication year - 2011
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.22108
Subject(s) - medicine , immunohistochemistry , cancer , pathological , pathology , gastric adenocarcinoma , messenger rna , oncofetal antigen , adenocarcinoma , real time polymerase chain reaction , gastroenterology , gene , biology , immunotherapy , biochemistry , tumor associated antigen
Background The aim of this study was to determine the expression of insulin‐like growth factor‐II messenger RNA (mRNA)‐binding protein‐3 (IMP‐3) and its clinical significance in gastric cancers, as well the prognostic value of its expression in the peritoneal lavage fluid after surgery. Methods IMP‐3 expression was examined by immunohistochemistry in 96 primary gastric tumors. IMP‐3 mRNA expression in peritoneal lavage fluid obtained at laparotomy was determine by real‐time quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR). Results Positive staining for IMP‐3 was observed in 74% (71/96) of the tumors. IMP‐3 expression in gastric tumors correlated significantly with worst overall survival (OS) and recurrence‐free survival. Multivariate analyses identified pathological N stage and IMP‐3 expression as significant independent prognostic factors for disease‐free survival. Eight (28%) of 36 peritoneal lavage samples were cytologically negative but positive for IMP‐3 mRNA expression by RT‐PCR. The OS of patients with IMP‐3‐positive peritoneal lavage was significantly worse than of those with negative expression. Conclusions IMP‐3 expression in primary gastric tumors was an independent poor prognostic factor. IMP‐3 mRNA expression in peritoneal lavage fluid was a predictor of recurrence after surgery in gastric cancer and a marker of poor prognosis. J. Surg. Oncol. 2012; 105:780–785. © 2011 Wiley Periodicals, Inc.