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Radiofrequency ablation of colorectal liver metastases induces an inflammatory response in distant hepatic metastases but not in local accelerated outgrowth
Author(s) -
Nijkamp Maarten W.,
Borren Alie,
Govaert Klaas M.,
Hoogwater Frederik J.H.,
Molenaar I. Quintus,
van Diest Paul J.,
Kranenburg Onno,
Rinkes Inne H.M. Borel
Publication year - 2010
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.21570
Subject(s) - medicine , radiofrequency ablation , cd8 , ablation , cancer research , pathology , colorectal cancer , immune system , cancer , immunology
Background Recently, we have shown in a murine model that radiofrequency ablation (RFA) induces accelerated outgrowth of colorectal micrometastases in the transition zone (TZ) surrounding the ablated lesion. Conversely, RFA also induces an anti‐tumor T‐cell response that may limit tumor growth at distant sites. Here we have evaluated whether an altered density of inflammatory cells could be observed in the perinecrotic (TZ) metastases compared to hepatic metastases in the distant reference zone (RZ). Methods RFA‐treated tumor‐bearing mice (n = 10) were sacrificed. The inflammatory cell density (neutrophils, macrophages, CD4 + T‐cells, and CD8 + T‐cells) of tumors in the TZ (TZ tumors) was compared to that in tumors in the RZ (RZ tumors). Sham‐operated, tumor‐bearing mice (n = 10) were analyzed simultaneously as controls (sham‐treated tumors). Results In RFA‐treated, tumor‐bearing mice RZ tumors contained a significantly higher density of neutrophils and CD4 + T‐cells, but not macrophages and CD8 + T‐cells compared to sham‐treated tumors. Notably, TZ tumors had a significantly lower density of neutrophils, CD4 + T‐cells, and CD8 + T‐cells, but not macrophages, when compared to RZ tumors. Conclusions The accelerated perinecrotic tumor outgrowth following RFA is associated with a reduced density of neutrophils and T‐cells compared to distant hepatic metastases. This may have implications for local tumor recurrence following RFA. J. Surg. Oncol. 2010; 101:551–556. © 2010 Wiley‐Liss, Inc.