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Transformation of pleomorphic adenoma to carcinoma ex pleomorphic adenoma of the parotid gland is independent of p53 mutations
Author(s) -
Gedlicka Claudia,
Item Chike B.,
Wögerbauer Markus,
Martinek Helga,
Heiduschka Gregor,
Erovic Boban M.,
Ch.Grasl Matthaeus,
Thurnher Dietmar
Publication year - 2010
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.21444
Subject(s) - pleomorphic adenoma , carcinoma ex pleomorphic adenoma , parotid gland , malignant transformation , pathology , adenoma , medicine , carcinoma , mixed tumor , salivary gland
Background and Objectives This retrospective study was performed to evaluate the status of p53 in pleomorphic adenomas and carcinomas ex pleomorphic adenoma in the parotid gland. As loss or mutation of p53 can cause malignant transformation, the possible degeneration of pleomorphic adenomas to carcinomas ex pleomorhic adenoma was investigated by mutational analysis. Methods Twenty‐five Patients including 14 patients with pleomorphic adenomas and 11 patients with carcinoma ex pleomorphic adenoma of the parotid gland were examined for p53 status. DNA was extracted out of paraffin‐embedded tissue and PCR was performed for the coding exons 2–11. Denaturing gradient gel electrophoresis (DGGE) was carried out for mutational analysis and DNA sequencing was performed in case of a suspected mutation. Results Fourteen pleomorphic adenomas and 11 carcinomas ex pleomorphic adenoma were screened for p53 status and potent mutations. Subsequent sequencing of the distinct exons showed no mutation. Conclusion We could not detect mutations of p53 neither in benign nor malignant parotid tumors and we therefore assume that p53 plays no role in the transformation from pleomorphic adenoma to carcinoma ex pleomorphic adenoma. J. Surg. Oncol. 2010;101:127–130. © 2009 Wiley‐Liss, Inc.