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Inverse relationship between the length of the EGFR CA repeat polymorphism in lung carcinoma and protein expression of EGFR in the carcinoma
Author(s) -
Suzuki Masaya,
Kageyama Shinji,
Shinmura Kazuya,
Okudela Koji,
Bunai Tomoyasu,
Nagura Kiyoko,
Igarashi Hisaki,
Kiyose Shinichiro,
Mori Hiroki,
Tao Hong,
Goto Masanori,
Takamochi Kazuya,
Mochizuki Takahiro,
Suzuki Kazuya,
Ohashi Riuko,
Ogawa Hiroshi,
Yamada Takeshi,
Niwa Hiroshi,
Tsuneyoshi Toshihiro,
Sugimura Haruhiko
Publication year - 2008
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.21130
Subject(s) - epidermal growth factor receptor , carcinoma , lung cancer , immunohistochemistry , copy number variation , gene , allele , microbiology and biotechnology , biology , cancer research , medicine , pathology , receptor , genetics , genome
Background and Objectives There is a CA dinucleotide repeat polymorphism in the first intron of the epidermal growth factor receptor ( EGFR ) gene. Our aim was to examine the relationship between the CA repeat length in lung carcinoma and EGFR mutation, EGFR gene copy number, and EGFR protein expression level in the carcinoma. Methods We examined 168 lung carcinomas for the length of the CA repeat polymorphism by PCR–polyacrylamide gel electrophoresis analysis, for EGFR mutations by sequencing analysis, for EGFR gene copy number by fluorescence in situ hybridization analysis, and for EGFR protein expression by immunohistochemical analysis. Results When the carcinomas were divided into two groups according to the length of their CA repeat polymorphism, the EGFR protein expression level was found to be significantly higher in the shorter allele group than in the longer allele group ( P = 0.0116), but its length was not associated with EGFR somatic mutations, high EGFR gene copy numbers, or clinicopathological factors, such as histological type or stage. Conclusions The results suggested that the length of the EGFR CA repeat polymorphism in lung carcinoma is inversely related with level of EGFR protein expression in the carcinoma. J. Surg. Oncol. © 2008 Wiley‐Liss, Inc.