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Estrogen sulfotransferase (SULT1E1) expression in benign and malignant human bone tumors
Author(s) -
Svoboda Martin,
Thalhammer Theresia,
Aust Sylvia,
Arrich Ferdi,
Assadian Ojan,
Toma Cyril D.
Publication year - 2007
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.20748
Subject(s) - medicine , osteosarcoma , estrogen , grading (engineering) , estrogen receptor , pathology , sarcoma , messenger rna , endocrinology , cancer , cancer research , breast cancer , biology , gene , ecology , biochemistry
Background and Objectives 17β‐estradiol regulates growth and differentiation in normal and malignant bone. E2 is inactivated to 17β‐estradiol‐sulfate through estrogen sulfotransferase (SULT1E1). Results In an explorative study, SULT1E1 mRNA expression was assessed in a broad range of samples from benign, primary and secondary malignant bone tumors. We detected SULT1E1 mRNA in 31/50 tumor samples (10/19 malignant, 6/13 benign tumors; 15/18 metastases). Significantly more SULT1E1‐positive samples were found in metastases than in primary bone tumors ( P = 0.019). Yet, there was no difference between malignant and benign primary tumors ( P = 0.718). SULT1E1 mRNA levels were not related to patients' age, gender, tumor location, stage, grading, and chemotherapy pretreatment. Relative SULT1E1 mRNA levels did not correlate with that of estrogen‐receptor alpha (ERα) as assessed by quantitative TaqMan PCR (10 malignant, 8 benign tissue samples). In the latter, ERα mRNA, but not SULT1E1 mRNA levels were significantly lower than in the malignant samples ( P = 0.006 and P = 0.71, respectively). Also, pronounced expression of SULT1E1 mRNA but not of ERα mRNA was observed in osteosarcoma (MG‐63, HOS) and Ewing's sarcoma (TC‐71) cells, while human osteoblasts and BMSC contained ERα but not SULT1E1 mRNA. Conclusion Frequent expression of SULT1E1 mRNA in various human bone tumors suggests that sulfonation might be important to control E2 levels and activity. J. Surg. Oncol. 2007;95:572–581. © 2007 Wiley‐Liss, Inc.