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E‐cadherin expression in early gastric carcinoma and correlation with lymph node metastasis
Author(s) -
Yi Kim Dong,
Kyoon Joo Jae,
Kyu Park Young,
Yeob Ryu Seong,
Soo Kim Hyun,
Kyun Noh Bok,
Hwa Lee Kyung,
Hyuk Lee Jae
Publication year - 2007
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.20732
Subject(s) - medicine , cadherin , immunohistochemistry , lymph node , oncology , metastasis , methylation , carcinoma , confidence interval , lymph node metastasis , pathology , dna methylation , cancer , gene expression , biology , gene , cell , biochemistry , genetics
Objective Abnormal expression of E‐cadherin plays an important role in the differentiation and progression of gastric carcinoma. However, the relationship between molecular changes in E‐cadherin and metastasis in early gastric carcinoma (EGC) is poorly understood. Materials and Methods Sixty cases of EGC with or without lymph node metastasis (30 node‐positive cases and 30 node‐negative cases) were investigated to evaluate hypermethylation status using bisulfate‐MSP and immunohistochemistry using antibody against E‐cadherin . Results Twenty‐seven (45.0%) of 60 primary EGCs exhibited methylation in the CpG island of E‐cadherin . Abnormal expression of E‐cadherin was significantly correlated with patient age, tumor size, Lauren classification, differentiation, and lymph node metastasis. Using multiple logistic regression analysis, two factors were independent, statistically significant parameters associated with lymph node metastasis: abnormal expression of E‐cadherin (risk ratio, 2.62; 95% confidence interval, 0.917–7.457; P < 0.05) and lymphatic invasion (risk ratio, 8.11; 95% confidence interval, 1.612–40.766; P < 0.05). Conclusion Our results suggest that methylation of E‐cadherin is a frequent, early event in gastric carcinoma progression, and is correlated significantly with downregulated E‐cadherin expression. Inactivation of E‐cadherin might be involved in metastasis in EGC and play an important role in microscopic differentiation. J. Surg. Oncol. 2007;96:429–435. © 2007 Wiley‐Liss, Inc.