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Expression of survivin and correlation with PCNA in osteosarcoma
Author(s) -
Wang Wenji,
Luo Huiying,
Wang Aiqin
Publication year - 2006
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.20507
Subject(s) - survivin , proliferating cell nuclear antigen , osteosarcoma , immunohistochemistry , malignancy , grading (engineering) , medicine , pathology , cancer research , biology , cancer , ecology
Background and Objectives Survivin is a new anti‐apoptosis gene with reported expression during fetal development and in cancer tissues. Proliferating cell nuclear antigen (PCNA) is essential for the replication of deoxyribonucleic acid (DNA). In this experiment, we examined the expression of survivin and PCNA in human osteosarcoma tissues and investigating whether survivin and PCNA expressions were correlated with histological grades, histological types, and patients' clinical characteristics. Methods Sixty‐eight osteosarcoma samples were divided into two groups based on the degree of tumor cell differentiation. Immunohistochemical staining was performed to analyze the expression of survinin and PCNA. Four histological types and patients' clinical characteristics were also recorded. Results There were abundant levels of survivin and PCNA immunoreactivity in the nucleus and/or cytoplasm of the osteosarcoma cells. All cells essentially revealed the cytoplasmic localization and the nuclear signals of survivin in same cases, while PCNA from the majority of cases predominantly showed nuclear expression. Scoring on the percentage of positive cells indicated that survivin expression was significantly associated with the PCNA‐labeling index (LI), which was correlated with the histological grades of osteosarcoma (χ 2 = 17.86 and χ 2 = 23.75, respectively). Conclusions The expression of survivin can be used as a useful indicator in grading the malignancy of osteosarcoma. J. Surg. Oncol. 2006;93:578–584. © 2006 Wiley‐Liss, Inc.