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Increased expression of matrix metalloproteinase‐2 and 9 and human papilloma virus infection are associated with malignant transformation of sinonasal inverted papilloma
Author(s) -
Katori Hideaki,
Nozawa Akinori,
Tsukuda Mamoru
Publication year - 2005
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.20386
Subject(s) - inverted papilloma , medicine , malignant transformation , pathology , dysplasia , papilloma , immunohistochemistry , in situ hybridization , hpv infection , carcinogenesis , matrix metalloproteinase , carcinoma , carcinoma in situ , cancer , biology , gene expression , cervical cancer , gene , biochemistry
Background and Objectives In this study, we identified matrix metalloproteinase (MMP)‐2 and 9 expression in exophytic papilloma (EP), inverted papilloma (IP) with dysplasia, IP with carcinoma, and invasive squamous cell carcinoma (SCC). We also compared the presence of MMP‐2 and 9 with human papilloma virus (HPV) infection. Methods The nasal tissue were stained with monoclonal antibodies to MMP‐2 and 9. The results were analyzed using quantitative immunohistochemical analysis. In situ hybridization studies for HPV DNA for 6/11, 16/18, and 31/33 were also performed on the tissue. Results Significant increase of MMP‐2 and 9 were observed in IP with moderate and severe dysplasia, IP with carcinoma, and invasive SCC compared to control nasal mucosa. Among IP, HPV 6/11‐positive was present in 41% and HPV 16/18‐positive was present in 31%. In HPV 6/11 and 16/18‐positive IP, significant increase of MMP‐2 and 9 were observed. Conclusions Precancerous lesions of IP exhibited elevated levels of MMP‐2 and 9 and these expression may be associated with early events in IP carcinogenesis. HPV infection would be an early event in a multistep process of malignant formation of IP. J. Surg. Oncol. 2006;93:80–85. © 2005 Wiley‐Liss, Inc.