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Instability of chromosome 8 as an indicator of aggressive tumor phenotype in pancreatic cancer
Author(s) -
Sato Norihiro,
Mizumoto Kazuhiro,
Maehara Naoki,
Shono Masaki,
Nagai Eishi,
Tanaka Masao
Publication year - 2001
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.1032
Subject(s) - chromosome instability , pancreatic cancer , genome instability , fluorescence in situ hybridization , cancer , chromosome , cancer research , pancreatic disease , motility , biology , cancer cell , phenotype , tumor progression , medicine , pathology , pancreas , genetics , gene , dna , dna damage
Background and Objectives Chromosomal instability is a common feature of pancreatic carcinoma, but its biological significance remains unclear. In this study, we investigated the association between chromosomal instability and biological aggressiveness in human pancreatic cancer cells. Methods Fluorescence in situ hybridization was performed to examine changes in chromosomal numbers in a total of 13 pancreatic cancer cell lines. We also assessed the potential for tumor aggressiveness within cancer cells by in vitro migration and invasion assay and by subcutaneous implantation into nude mice. Results Chromosomal instability, characterized by numerical variations in copy numbers of chromosome 8, was observed in most cell lines, and the magnitude of instability was correlated well with both motility ( P < 0.001) and invasion rate ( P < 0.001) of these cells. Furthermore, a significant positive correlation existed between chromosome instability and tumor growth in vivo ( P < 0.01). Conclusions These results suggest that the increased level of chromosomal instability may play a critical role in the development of aggressive tumor phenotype during pancreatic cancer progression. J. Surg. Oncol. 2001;76:181–187. © 2001 Wiley‐Liss, Inc.