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Quantitative analysis of heparanase messenger RNA expression in hepatocellular carcinoma
Author(s) -
Ikeguchi Masahide,
Ueta Tsuyoshi,
Yamane Yoshiaki,
Hirooka Yasuaki,
Kaibara Nobuaki
Publication year - 2002
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.10163
Subject(s) - heparanase , hepatocellular carcinoma , messenger rna , medicine , reverse transcriptase , basement membrane , real time polymerase chain reaction , cancer research , rna , pathology , cancer , metastasis , biology , gene , biochemistry
Background and Objectives In the process of tumor invasion, cancer cells secrete enzymes including heparanase that degrade extracellular matrices and basement membranes of blood vessels. In the present study, the clinical importance of enhanced expression of heparanase messenger RNA (mRNA) in hepatocellular carcinoma (HCC) was evaluated. Methods Fresh tumor and noncancerous liver tissue adjacent to a tumor were obtained from 50 patients with HCC immediately after hepatic resection. The expression levels of heparanase mRNA were analyzed quantitatively, using the real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR) method, and were compared with clinicopathological findings. Results Relative heparanase mRNA expression level in HCC was significantly lower than that of noncancerous liver tissue ( P < 0.001). Tumor heparanase expression did not correlate with tumor differentiation, tumor stage, or patient prognosis. Conclusions Enhanced heparanase mRNA expression may not be a good biological marker in HCC. J. Surg. Oncol. 2002;81:148–154. © 2002 Wiley‐Liss, Inc.